RB EK EGF Epidermal Growth Factor Recombinant Bovine Enterokinase Skin Care

RB EK EGF Epidermal Growth Factor Recombinant Bovine Enterokinase Skin Care

Product Name: Recombinant Bovine Enterokinase ( rb-EK)

Formulation: Lyophilized from 50 mM Tris-HCl, pH8.0

Mol. Wt.: 26.2 kDa, 30 kDa by SDS-PAGE analysis

Theoretical pI: 5.20

Activity: ≥40 KU/mg

Purity: ≥95% by SDS-PAGE analysis

Endotoxin: ≤5 EU/mg

Resources: Escherichia coli (E. coli)

Storage Condition: -20 ^oC

Storage Duration: 3 years

Unit Definition: 1 unit is defined as the amount of rb-EK that will digest 50 μg of 1mg/ml fusion protein to 95% completion in 16 h at 25^oC in 50 mM Tris-HCl, pH 8.0.

Description:

Enterokinase is a serine protease that can specifically cleave the sequence Asp-Asp-Asp-Asp-Lys after the lysine residue . It applies for digesting fusion proteins to release the fusion partner or tag from the target protein.

Native enterokinase is composed of a 115 kDa heavy chain as a membrane anchor and a 35 kDa light chain as the catalytic subunit. Recombinant bovine enterokinase is a specially prepared recombinant enzyme, consisting of the catalytic subunit of the holoenzyme. This subunit is expressed and purified from E.coli, yielding an enzyme with higher specific activity. This results in more efficient cleavage using less enzyme.

Note:

1. Rb-EK is known to be inhibited by >2 M Urea, >250 mM NaCl, >20 mM β-ME, >0.1% SDS, or >50 mM imidazole. Dialyze your fusion protein to remove the inhibitors prior to digestion with rb-EK.

2. Avoid using phosphate buffer.